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Gill Snip/Biopsy

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Gill Snip/Biopsy

Post by Admin on Tue Aug 23, 2011 3:57 am

Only 2mm from the tips of Primary Lamellae (fringes) needs to be snipped for microscopy.

http://books.google.co.uk/books?id=zdXW8NjV3IQC&pg=PA28&lpg=PA28&dq=how+to+take+a+gill+biopsy&source=bl&ots=u2qQzIApzw&sig=tDI6f-tVKKfBMkzaTL4_ojLSpaI&hl=en&ei=i4JSTu39F8nChAfHnbXuBg&sa=X&oi=book_result&ct=result&resnum=10&ved=0CFYQ6AEwCQ#v=onepage&q&f=false
http://www.usakoi.com/profile/news/read/id/51
http://www.flickr.com/photos/suewaters/3181375423/
http://www.fishdoc.co.uk/microscope/micro06.htm

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Re: Gill Snip/Biopsy

Post by DaveB on Tue Aug 23, 2011 5:37 pm

Mio

Nice links good of you to find the time. I have one question , After placing gill biopsy onto the slide, adding drop of water and cover slip. Is it necessary to squeez the sample, making thin as possible. I have always done this wiith mucous scrapes. Which is something I was shown years ago.

Best regards Dave

P.S. Another question if I may. What are you guys using to sterilize equipment ie alcohol and where are you getting it from? I have an idea of using a old vegatable steamer, but not sure if it would work
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Re: Gill Snip/Biopsy

Post by Admin on Tue Aug 23, 2011 9:32 pm

DaveB wrote:Mio

Nice links good of you to find the time. I have one question , After placing gill biopsy onto the slide, adding drop of water and cover slip. Is it necessary to squeez the sample, making thin as possible. I have always done this wiith mucous scrapes. Which is something I was shown years ago.
Best regards Dave

P.S. Another question if I may. What are you guys using to sterilize equipment ie alcohol and where are you getting it from? I have an idea of using a old vegatable steamer, but not sure if it would work

There are differing opinions here Dave ...

Some suggest `squashing` the lamellae sample and others recommend using a `welled` slide to keep the specimen intact scratch


Personally I don`t squash `Gill Snip` samples myself. Arrow
https://docs.google.com/viewer?a=v&q=cache:D6-GGh2rjCIJ:www.koivet.com/downloads/WETLABPresentation.pdf+taking+a+gill+gill+snip+fishdoc&hl=en&gl=uk&pid=bl&srcid=ADGEESj-kbo4C90czV4R4E7L2QWSPM0JJc_BNDm_xCuYTw_KqNlrgoy45CnBN5RWNkorgJYQrckRFY7Gt8Php3Ry1-Bk_thWqgxj1E5CvAXM_e20-391Dp1SkdKeiPHRyyOjOqc5JsSX&sig=AHIEtbRGCgsrxpvfVgDoleb_CeQS6kSrGQ

Re sterilizing scissors etc ... I place mine in a bowl of boiling water until it cools then use them once .. wash these well place them back in the sealed plastic bag until needed again.

I use these to wipe the Scope and slides after washing just prior to using them Arrow http://www.ebay.co.uk/sch/sis.html?_nkw=100+ANTI+STATIC+IPA+ISOPROPYL+ALCOHOL+CLEANING+WIPES

Nancy might have more useful tips re sterilizing equipment etc given she works in a Lab Very Happy

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Re: Gill Snip/Biopsy

Post by DaveB on Wed Aug 24, 2011 4:35 am

Mio Thanks for the reply. is it still possible to focus on the lamallea when looking for costia at 400x. that is if costia do infact infect the gills. Best REgards Dave

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Re: Gill Snip/Biopsy

Post by Admin on Wed Aug 24, 2011 5:12 am

DaveB wrote:Mio Thanks for the reply. is it still possible to focus on the lamallea when looking for costia at 400x. that is if costia do infact infect the gills. Best REgards Dave

Most Parasites prefer `Gills` Dave Twisted Evil even harmless species are not averse to taking up residence there if lesions are in Gill tissue.

Dr Thomas L. Wellborn Jr image below Arrow not certain of the magnification but it looks 400X.



More Ichthyobodo Necator aka Costia in Gill Filament Images here Arrow

You will need to view 4-600 X Magnification to get a good look at the Critter IMO.

http://www.google.co.uk/search?q=costia+images&hl=en&prmd=ivns&tbm=isch&tbo=u&source=univ&sa=X&ei=g-lTTsn4L8238QO55M3XBQ&ved=0CBwQsAQ&biw=1040&bih=668#hl=en&tbm=isch&sa=1&q=costia+in+gill+filaments+images&oq=costia+in+gill+filaments+images&aq=f&aqi=&aql=&gs_sm=e&gs_upl=7387l8209l8l9418l4l4l0l1l0l0l196l386l1.2l3l0&bav=on.2,or.r_gc.r_pw.&fp=508034f7331e7fdc&biw=1280&bih=666


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Re: Gill Snip/Biopsy

Post by DaveB on Wed Aug 24, 2011 6:54 pm

Mio Thanks again. your links lead to some great videos

Best Regards Dave

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Re: Gill Snip/Biopsy

Post by Admin on Wed Aug 24, 2011 7:02 pm

DaveB wrote:Mio Thanks again. your links lead to some great videos

Best Regards Dave

Costia ( Icthyobodo Necator ) is readily identifiable due to the `movement` Dave ... It flips and tumbles at remarkable speed.

Doc Johnsons Parasite Video page ( one of the the clips you might have spotted ) here Arrow http://www.koivet.com/movies/index.html

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